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Even when transcription per se might have been implicated inside processes, there is now plentiful evidence that the cohesin advanced plays the new major part inside the cycle extrusion. To evaluate the newest joining of cohesin at that designed locus, i performed Processor-seq of your cohesin parts RAD21. The fresh endogenous RAD21 protein is actually tagged having a twin Strep-tag (RAD21TST) and you may Processor-seq is performed within the R2-installation and you can WT erythroid cells Fig. A peak of cohesin sometimes appears from the R2 installation web site inside R2-insertion erythroid tissues (arrow one in Fig. 3A), and that represents the brand new discover chromatin website identified thanks to ATAC-seq.